Journal: Natural Products and Bioprospecting

Article Title: Harziachalasins A–G, polycyclic-fused cytochalasins from the endophytic fungus Trichoderma harzianum MLJ-4 with HIV latency reversal activity

doi: 10.1007/s13659-025-00572-1

Figure Lengend Snippet: Compound 4 reprograms transcriptomes in the HIV latent cell model with experimental validation of NF- κ B pathway activation. A Volcano plot shows significantly different expressed genes in J-Lat 10.6 cells treated with or without 10 μM of 4 for 24 h. B GSEA enrichment result of the Hallmark gene set. C Positive enrichment of the Hallmark TNF- α signaling via NF- κ B gene set in compound 4 -treated cells. D I κ B α degradation and phosphorylation induced by 10 μM compound 4 . E Dose-dependent I κ B α degradation and phosphorylation induced by 4 after 15-min treatment. F PDTC attenuates the HIV latency reversal effect of 4 . G Bubble plot depicting enriched Reactome pathways results. H Results from Disease Ontology (DO) enrichment analysis were demonstrated by bubble plot

Article Snippet: Cells treated with compound 4 under indicated conditions were collected by centrifugation (800 rpm, 25 °C, 5 min) and lysed using IP buffer (Beyotime) containing phosphatase inhibitor (Selleck).

Techniques: Biomarker Discovery, Activation Assay, Phospho-proteomics